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Xenbase Image ID: 134850

Fig. 6. Lineage tracing reveals the formation of dorsal and anterior structures by the progeny of a single blastomere injected with Xdsh mRNA. Cleavage-stage (8-32 cells) embryos were coinjected with 0.4 ng of Xdsh mRNA and 0.2 ng of b-gal RNA or with b-gal RNA alone. After 2 days of development embryos were fixed, and stained for b-gal activity. (A) Normal embryos were injected at the 8- to 16-cell stage into a ventrovegetal blastomere with b-gal RNA (two embryos on the top) or with b-gal and Xdsh mRNAs (bottom). (B,C) UV-treated embryos rescued by Xdsh RNA and coinjected with b-gal RNA. (B) Staining is mainly in the pharyngeal endoderm and head mesenchyme of the fully rescued embryos. A control embryo injected with b-gal RNA only is shown at the top. (C) In the partially rescued embryos (0.05-0.1 ng of Xdsh RNA injected), the staining is in the notochord and pharyngeal endoderm. (D-F) Lineage tracing at the 32-cell stage. Embryos were injected into D4 (tier 4) vegetal blastomere with b-gal RNA (D) or with b-gal and Xdsh mRNAs (E). (F) Xdsh and b-gal RNAs were microinjected into C4 (tier 3) subequatorial blastomere. Staining is mainly in the notochord and anterior mesoderm.

Image published in: Sokol SY et al. (1995)

Copyright © 1995. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.

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