XB-IMG-134860
Xenbase Image ID: 134860
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Fig. 4. Immunohistochemical analysis of animal caps injected with increasing quantities of Xbra RNA. a-smooth muscle actin and somitic
muscle were defined with monoclonal antibodies a-SM actin (Saint-Jeannet et al., 1992) and 12/101 (Kintner and Brockes, 1984), respectively.
Embryos at the 1-cell stage were injected with increasing amounts of Xbra RNA. Animal caps were dissected from these embryos at the midblastula
stage and cultured to stage 47, when they were fixed and embedded in acrylamide prior to sectioning at 10 mm (see Cunliffe and Smith,
1994). Sections were immunostained with a-SM actin (A-C) and 12/101 (D-F), and a FITC-conjugated goat anti-mouse secondary antibody.
(A,D) Uninjected animal caps. (B,E) Animal caps from embryos injected with 0.5 ng Xbra RNA. (C,F) Animal caps from embryos injected
with 4 ng Xbra RNA. Results are summarised in Table 2. Scale bar in F is 50 mm, and also applies to A to E. Image published in: O'Reilly MA et al. (1995) Copyright © 1995. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View |