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Fig. 5. Co-expression of zebrafish goosecoid and ntl RNAs does not induce cardiac (muscle-specific) actin expression in Xenopus animal caps. Embryos at the 1-cell stage were injected with RNA encoding zebrafish goosecoid (1 ng) and/or ntl (0.4 ng) and/or Xenopus noggin (100 pg) as indicated. Animal caps were dissected at the mid-blastula stage and cultured to stage 28, when they were analyzed for expression of actin genes by RNAase protection. 0.4 ng ntl did not induce expression of muscle-specific actin genes, but did cause formation of ventral mesoderm as judged by morphological and histological criteria (not shown). Co-expression of noggin and ntl, but not goosecoid and ntl, induces expression of muscle-specific actin RNA.

Image published in: O'Reilly MA et al. (1995)

Copyright © 1995. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.

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