Xenbase Image ID: 134927

Fig. 4. At stage 7, mixing of the D1.1 clone is primarily with its contralateral neighbor. One D1.1 blastomere was labeled with FDA (green), the contralateral D1.1 blastomere was labeled with TRDA (red). Transverse section, dorsal is to the top. Bar equals 200 mm in all color photomicrographs. Fig. 5. At stage 8, descendants of A4 (red) were found one or two cell diameters on the dorsal side of the animal pole (line), while the animalmost descendants of A1 (green) no longer reached the animal pole. Sagittal section, dorsal is to the right. Fig. 7. At stage 9, the A4 clone (green) has moved into the dorsal part of the animal cap. Its deep cells underlie more superficial cells of the B4 clone (red) in the blastocoel roof. Sagittal section, dorsal is to the left, animal pole is marked by line. Fig. 8. At stage 9 both the B1 clone (red) and the C1 clone (green) have moved into the vegetal marginal zone. A few descendants of C1 can be found three or four cell diameters within the clone of B1. Parasagittal section, dorsal is to the right. Dotted line indicates level of the blastocoel. Fig. 9. At stage 10, the A4 clone (green) has spread over most of the blastocoel roof. The B4 clone (red) extends from the animal cap to the noninvoluting marginal zone (solid arrow), contributing some deep cells to the involuting mesoderm (open arrow). Sagittal section, dorsal is in the lower right corner. Animal pole is marked by line. Fig. 10. At stage 11, the clones of A1 (green) and B1 (red) are distinct in preinvolution mesoderm, but mix as they approach the site of invagination (arrow) and enter the postinvolution mesoderm. Sagittal section, dorsal is to the top. Fig. 11. At stage 10, the B1 clone (red) occupies the dorsal blastopore lip to the site of invagination (arrow). The C1 clone (green) stretches from the yolk plug to the floor of the blastocoel. Figs 12, 13. Two examples of stage 10 embryos in which the B1 clone (red) is the primary contributor to the marginal zone of the dorsal blastopore lip, but members of the C1 clone (green) also make a small contribution. Arrows mark the site of invagination. Orientation is the same as in Fig. 11. Fig. 14. At stage 12.5, the A1 clone (green) has reached the blastopore lip (arrow) and contributes extensively to the entire neural ectoderm. The B1 clone (red) extends throughout the anterior/posterior extent of both neural and mesodermal layers. a, anterior; p, posterior. Sagittal section dorsal is to the top. Fig. 15. At stage 12, the A4 clone (green) and the B4 clone (red) extend across the ventral epidermis from anterior (a) to posterior (p). The ventral blastopore lip is at the upper left edge of the micrograph (arrow). The clones intermix extensively in the posterior half of the deep layer. Sagittal section dorsal is to the top. Image published in: Bauer DV et al. (1994) Copyright © 1994. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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