Xenbase Image ID: 134964

sigFig. 3. Validation of xbra and other genes affected by ext1 knockdown. (A) Selected genes identified by microarray were validated using Q-RT-PCR. Embryos injected with 20 ng of EXT1 MO or EXT1 MIS were collected at stage 10.5 for RNA extraction and Q-RT-PCR. (B) In situ hybridization shows differences in xbra expression in control vs ext1 morphant embryos. Results are representative of over 2/3 of embryos from 3 independent experiments. (C) Q-RT-PCR assays for expression of the xbra target genes eFGF and Wnt11. Relative fold enrichment of each target gene was calculated after normalization with the housekeeping gene ornithine decarboxylase (ODC) using the δδCt method. Bars represent mean ± S.D. N=3 independent experiments. *: p < 0.05. Image published in: Shieh YE et al. (2014) Copyright © 2014. Reproduced with permission of the Publisher, University of the Basque Country Press. Gene Synonyms Species Stage(s) Tissue tbxt.S bra, brachyury, ntl, t, t-a, t-b, X-bra, Xbra, Xbrachyury X. laevis Throughout NF stage 11 mesoderm Image source: Published Larger Image Printer Friendly View

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