Xenbase Image ID: 134966

Fig. 5. Establishment of dorsal- or ventral-specific patterns of gene expression in ext1 morphant embryos. Embryos were injected with 20 ng of EXT1 or mispair MO. In situ hybridization of vent1 (A,B); vent2 (C,D); not1 (E,F); and otx2 (G,H). Expression patterns shown are representative of at least 2/3 of embryos from 3 independent experiments. (I) Q-RT-PCR assays of organizer-specific gene expression in ext1 MO or mispair MO embryos. Relative –fold changes were evaluated as described in Fig. 3. Bars represent mean ± S.D. N=3 independent experiments. *: p < 0.05. Image published in: Shieh YE et al. (2014) Copyright © 2014. Reproduced with permission of the Publisher, University of the Basque Country Press. Gene Synonyms Species Stage(s) Tissue otx2.S otx-2, otx2-a, otx2-b, otxA, Xotx-2, Xotx2 X. laevis Throughout NF stage 11 upper blastopore lip not.S flh, floating head, not-a, not-b, not1, noto, Xnot, Xnot-2, Xnot1, Xnot2, Znot X. laevis Throughout NF stage 11 upper blastopore lip ventx2.2.L vent-2, vent2, Xom, xvent-2, xvent2 X. laevis Throughout NF stage 11 marginal zone ventral marginal zone ventro-lateral marginal zone ventx1.L posterior-ventral 1, PV.1, ventx1.1, ventx1.1-a, ventx1.1-b, Xvent-1b X. laevis Throughout NF stage 11 marginal zone ventral marginal zone ventro-lateral marginal zone Image source: Published Larger Image Printer Friendly View

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