Xenbase Image ID: 135038

Fig. 3. In situ hybridization to sectioned tissue from oocytes and embryos. Middle and late stage oocytes were oriented during embedding such that sections cut through the animal-vegetal axis. Early stage oocytes were randomly oriented during embedding. (A) Early stage I-II oocytes (small arrows) have Xwnt-11 mRNA distributed uniformly in the cytoplasm. Middle stage III-IV oocytes (large arrows) are beginning to localize Xwnt-11 mRNA at one pole of the oocyte. The germinal vesicle is visible as a dark circle in the middle of each oocyte. The sections shown in B, C and D are all oriented with the animal pole facing up and the vegetal pole facing down. (B) Late stage V-VI oocyte shows that Xwnt-11 mRNA is localized to the vegetal cortex and to patches in the vegetal cytoplasm. The germinal vesicle is in the animal hemisphere. (C) Unfertilized egg shows that Xwnt-11 mRNA is diffusely localized to the vegetal hemisphere. (D) 8-cellstage embryo shows that Xwnt-11 mRNA remains diffusely localized to the vegetal hemisphere. The horizontal cleavage plane of the third cell division (arrowheads) confines the mRNA to vegetal blastomeres. Image published in: Ku M and Melton DA (1993) Copyright © 1993. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue wnt11b.L wnt-11, wnt11, Xwnt-11, xwnt11 X. laevis Sometime during unfertilized egg stage to NF stage 8 zygote cortex vegetal vegetal pole vegetal hemisphere Image source: Published Larger Image Printer Friendly View

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