Xenbase Image ID: 135039

Fig. 4. Whole-mount in situ hybridization with a Xwnt-11 probe on embryos of different stages. (A) Late blastula (stage 9), vegetal view, dorsal is up. Staining is present in a broad arc in the marginal zone of the embryo on the dorsal side. This arc is much broader than the organizer. (B) Early gastrula (stage 10.5), vegetal view, dorsal is up. The marginal zone staining has extended laterally and ventrally. Highest levels of expression are on the dorsal side in an area roughly corresponding to the organizer (60°around the dorsal mid-line). The lateral and ventral marginal zone staining does not extend down to the blastopore lip. (C) Late gastrula (stage 12.5), vegetal view, dorsal is up. A ring of staining around the closing blastopore is present. The few dorsal-most staining cells extending out along the dorsal midline probably include presumptive posterior neural plate and archenteron roof cells. (D) Late neurula (neural folds have closed), view from posterior-ventral side, dorsal is up. The blastopore is almost completely closed and is visible only as a small slit. Xwnt-11 expressing cells are present both dorsal and ventral to the blastopore. (E) Early tailbud (stage 22-23), anterior to the right, dorsal is up. The predominant staining is around somite nuclei, which are aligned vertically in each somite, and in the first branchial arch. (F) Tadpole (stage 28), anterior to the right, dorsal is up. Xwnt-11 staining is present around the somite nuclei, which are aligned vertically in each somite, and in the first branchial arch. Scale bars all represent 500 μm. Image published in: Ku M and Melton DA (1993) Copyright © 1993. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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