Xenbase Image ID: 135040

Fig. 5. Sections of whole-mount embryos hybridized with Xwnt-11 antisense probe. All sections were oriented during embedding such that the sections cut through the dorsal and ventral midline. (A). This section is late stage 9 or early stage 10, and Xwnt-11 mRNA (between arrowheads) is beginning to appear in a region that will later be above the dorsal blastopore lip. No involution has occurred yet at this stage, and the mRNA is present in surface and deep layer cells. The embryo is oriented with the animal pole up and the vegetal pole (vg) down. The blastocoel floor is marked (bf). Fissures are an artifact of sectioning. (B) Mid gastrula (stage 12) shows Xwnt-11 mRNA (between arrowheads) in the dorsal blastopore lip (area below dotted line). Cell boundaries are difficult to discern, but the positively staining cells probably include both surface epithelial cells and deeper cells but not cells that have already involuted. The archenteron is marked (a). Image published in: Ku M and Melton DA (1993) Copyright © 1993. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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