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XB-IMG-135041

Xenbase Image ID: 135041


Fig. 6. Histological sections of UVventralized eggs injected with Xwnt-11 mRNA. UV-ventralized embryos, UV embryos injected with Xwnt-11 mRNA and normal embryos were fixed at stage 26-28, sectioned and stained to determine what structures had formed. (A) UVventralized embryo, cross section. (B) UV embryos injected with Xwnt-11 mRNA. Mid-trunk cross section, dorsal is up. (C) Higher magnification of a UV embryo injected with Xwnt-11 mRNA. The neural tube (nt) consists of a dark- and a palestained region. There is no lumen in this section, but other sections sometimes do show a lumen. The pale-stained region is the ‘marginal zone (mz)’, where axon tracts are located. There is one large marginal zone in the ventral midline (compare with Fig. 6E). There is also one large mass of muscle (m) that extends across the midline. (D) Normal embryo. Mid-trunk cross-section, dorsal is up. (E) Higher magnification view of a normal embryo. Neural tube (nt) and notochord (n) are visible. One of the two files of somitic muscle on each side of the notochord is marked (m). Note the two lateral pale-stained ‘marginal zones’ (mz) separated by ventral floor plate cells. The scale bars in A, B and D) represent 100 μm and in D and E represent 50 μm.

Image published in: Ku M and Melton DA (1993)

Copyright © 1993. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.

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