XB-IMG-135042
Xenbase Image ID: 135042
|
|
Fig. 7. Whole-mount immunohistochemistry of Xwnt-11-injected embryos. UV-ventralized embryos (A,D), UV embryos injected with
Xwnt-11 mRNA (B,E) and normal embryos (C,F) were fixed at stage 26-28, and whole-mount immunohistochemistry was done with
tissue-specific antibodies. UV-ventralized embryos have neither notochord nor muscle. UV embryos injected with Xwnt-11 mRNA have
somitic muscle but no notochord. Normal control embryos have both muscle and notochord. (A-C) Incubated with the notochord-specific
monoclonal antibody (mAb) Tor 70 (Kushner, 1984; Bolce et al., 1992). (D-F) Incubated with the muscle-specific monoclonal (mAb)
antibody 12101 (Kintner and Brockes, 1984). (A) UV-ventralized embryo plus Tor 70 mAb. There are no Tor 70-positive cells. (B) UV
embryo injected with Xwnt-11 mRNA plus Tor 70 mAb. There are a few scattered Tor 70-positive cells but no organized notochord is
present. (C) Normal embryo plus Tor 70 mAb. An extensive notochord is present along the dorsal axis. (D) UV-ventralized embryo plus
12101 mAb. There are no 12101-positive cells. (E) UV embryo injected with Xwnt-11 mRNA plus 12101 mAb. Extensive somitic muscle
is present along the dorsal axis. (F) Normal embryo plus 12101 mAb. Somitic muscle is present. Scale bars all represent 100 μm. Image published in: Ku M and Melton DA (1993) Copyright © 1993. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View |