XB-IMG-135050
Xenbase Image ID: 135050
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Fig. 3.Expression of XMHCa Xenopus embryos. The prevalence of
cardiac actin (A), XMyoD (B) and XMHCa transcripts (C) in early
embryos was analysed by RNAase protection assay. Myotomeenriched
dorsal fragments were dissected from tailbud or early
tadpole embryos and compared with ventral pieces that included the
heart anlage (D). P, undigested probe; t, 10 mg tRNA control; lanes
2, 4, 6, 8 and 10, dorsal fragments (stages 26, 28, 30, 32 and 34,
respectively); lanes 3, 5, 7, 9 and 11, ventral fragment from the same
embryos. Total RNA from four fragments was analysed in each lane.
These comprised approximately similar amounts, as determined
using the EF1a probe (data not shown). Full-length, protected
fragments for each probe are indicated. The cardiac actin probe gives
several partial protection products resulting from cross hybridisation
with cytoskeletal actin transcripts. A prominent, dorsal-specific band
(open triangle) is obtained with the XMHCa probe. Image published in: Logan M and Mohun T (1993) Copyright © 1993. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View |