XB-IMG-135052
Xenbase Image ID: 135052
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Fig. 5. Induction of XMHCa expression in animal pole explants.
Activin-induced animal pole explants were assayed for the
presence of cardiac actin (A), XMyoD (B) and XMHCa
transcripts (C) using an RNAase protection assay. P, undigested
probe; lane 1, 10 mg tRNA; lanes 2-8, total RNA from induced
explants cultured until sibling embryos reached stages 18, 22, 26,
30, 34, 38 and 42, respectively; lane 9, 3 mg of total tadpole RNA
(stage 42). Full-length, protected fragments for each probe are
indicated, as is the myotome-specific band (open triangle)
obtained with the XMHCa probe. The equivalent of four explants
were analysed in each assay and contained similar amounts of
total RNA, as judged by the level of EF1a transcripts (C). Image published in: Logan M and Mohun T (1993) Copyright © 1993. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View |