XB-IMG-135053
Xenbase Image ID: 135053
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Fig. 6. XMHCa expression in explants is induced by activin A
but not by bFGF. Blastula animal pole explants were induced with
bFGF or activin A and cultured until control embryos reached
stage 42. Total RNA was assayed for cardiac actin (A) and
XMHCa mRNA (B) by RNAase protection. P, Undigested probe;
lane 1, 10 mg tRNA; lane 2, uninduced explants (control for
bFGF); lanes 3-7, explants induced with 10, 20, 40, 80 and 120
units/ml of Xenopus recombinant bFGF; lane 8, uninduced
explants (control for activin); lanes 9-11, explants induced with 8,
32 and 80 units/ml of human recombinant activin A. The
equivalent of four explants were analysed in each assay. The level
of cardiac actin mRNA detected should be normalised by
reference to the signal obtained for the cross-hybridising
cytoskeletal actin transcripts (A). In the XMHCa assays (B), the
EF1a probe was included to monitor the relative amounts of
explant RNA. Image published in: Logan M and Mohun T (1993) Copyright © 1993. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View |