XB-IMG-135057
Xenbase Image ID: 135057
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Fig. 3. The GATA-4 gene is transcribed in heart, gonads and
endodermal derivatives. Northern blots containing 10 mg of total
cellular RNA were hybridized first to a GATA-4 cDNA probe.
After exposure, the blots were stripped and reprobed using an EF-
1a cDNA probe to demonstrate RNA integrity. RNA standards
(not shown) were used to determine an approximate size of the
GATA-4 message as 1.8 kb. The arrow indicates a faint but
reproducible slightly larger message found in ovary and testis.
Additional minor higher molecular weight RNA species are
evident in tissues that express high levels of GATA-4. Small
intestine was divided into duodenum (f, female; m, male) and a
distal or proximal (prox) segment of the ileum. Additional tissues
were derived from 2-week-old tadpoles (tp). In the experiment
shown, transcription in the lung was unusually high. Also,
although not obvious in the reproduction, the GATA-4 message
was consistently detected in liver. Image published in: Kelley C et al. (1993) Copyright © 1993. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View |