XB-IMG-135062
Xenbase Image ID: 135062
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Fig. 8. Quantitative RT/PCR analysis of GATA-4 RNA in embryo explants. (A) The GATA-4 gene is activated in the presumptive
mesoderm and vegetal explants. Embryos were dissected at stage 8 and explants were incubated in 0.1´ MBS until 30 hours
postfertilization. RNA was then prepared and analyzed as described in Materials and
Methods. Multiplex PCR employed primers specific for either GATA-4 cDNA or ornithine
decarboxylase (ODC) cDNA. The latter transcripts were co-analyzed as an internal control.
A, animal caps; V, vegetal explants (three independent experiments are shown); M,
presumptive mesoderm; E, whole embryo; NT, no RT included control. (B) The GATA-4
gene is activated in both the ventral marginal zone (VMZ) and the dorsal marginal zone
(DMZ). Marginal zones were dissected at stage 12.25 and incubated in buffer alone until
control embryos reached the indicated stages. RNA was analyzed as above. Two
independent experiments are shown for each VMZ and DMZ explant. In the control lane
reaction, RT was omitted. Image published in: Kelley C et al. (1993) Copyright © 1993. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View |