XB-IMG-135075
Xenbase Image ID: 135075
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Fig. 3. Effects of overexpressing the control (HAV0/) and dominant negative FGF receptor (XFD) constructs on brachyury expression. (A)
One blastomere at the two-cell stage was injected with either HAV0/ (left) or XFD (right) RNA. At the mid gastrula stage (st. 11), the
embryos were fixed and subjected to whole-mount in situ hybridization with a Xenopus brachyury (Xbra) probe (brown stain). After
staining for Xbra some embryos were then stained with the FGF receptor antibody. The embryo in B was injected with HAV0/ RNA and
the embryo in C was injected with XFD RNA. In these panels the stain for brachyury is blue while the FGF receptor protein stain is redbrown.
(D, E) The two dorsal blastomeres of four-cell stage embryos were co-injected with HAVø and b-galactosidase RNA (D) or XFD
and b-galactosidase RNA (E). At the mid gastrula stage (st. 11) the embryos were fixed and stained for b-galactosidase activity (blue
stain) and then subjected to in situ hybridization with the brachyury (Xbra) probe (brown stain). The embryos in D and E are oriented
with the dorsal side toward the top of the page. To emphasize the dorsal side, the embryos were not cleared and tilted ventrally, resulting
in the bleached appearance of the ventral side from reflected light. Scale bars, 250 um. Image published in: Amaya E et al. (1993) Copyright © 1993. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.
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