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Figure 5. Xhh expression is also activated during TH induced intestinal metamorphosis. Stage 56 tadpoles were treated with 5 nM T3 for various times as indicated in days. RNA was isolated from the intestine for Northern blot analysis using either Xhh or rpL8 as a probe. 28S and 18S ribosomal markers are indicated on the left. It is interesting to note that in the absence ofexogenous TH it normally takes 8 days for stage 56 tadpoles to reach stage 60 when Xhh mRNA levels are upregulated (Fig. 4), 11 days to reach the peak levels of Xhh mRNA (stage 62), and 3 weeks to reach stage 66 when Xhh expression is repressed again.

Image published in: Stolow MA and Shi YB (1995)

Copyright © 1995. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.

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