XB-IMG-135168
Xenbase Image ID: 135168
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Figure 6. Xhh is a directTH response gene. (A) Kinetics ofT3 induction. Stage
52-54 tadpoles were treated with 5 nM T3 for various times as indicated in
hours. RNA was isolated from intestine for RNA blot hybridization using full
length Xhh orrpL8 as a probe. (B)TH activation ofthe Xhh gene is independent
of new protein synthesis. Protein synthesis inhibitors cyclohexamide and
anisomycin (CHX) were added to stage 52-54 tadpoles 1 h before the addition
of 50 nM T3. The treatment was continued for another 12 h before RNA was
isolated from intestine for RNA blot analysis using Xhh or rpL8 as a probe.
Ribosomal markers, 28S and 18S, are indicated on the left. Image published in: Stolow MA and Shi YB (1995) Copyright © 1995. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View |