Xenbase Image ID: 135224

Figure 1. Xwnt-8 is expressed in all mesodermal cells except those of the Spemann organizer field. Whole-mount in situ hybridization studies in Xenopus embryos. (A) Early gastrulae (stage 10) hybridized with Xwnt-8 antisense (lower embryo) and sense (upper embryo) probes. Arrows denote the position of the dorsal lip of the blastopore. (B) Mid-gastrulae (stage 11, lower embryo) and late gastrulae (stage 12, upper embryo). Arrows denote lateral cells that express Xwnt-8 and are undergoing convergent extension movements toward the animal pole and dorsal midline. (C) Transverse section of a stage-20 neurula showing Xwnt-8 signal in lateral and ventral plate mesoderm. (Inset) Characteristic staining observed when embryos are treated for a longer period with proteinase K before hybridization. Note that the signal is more intense, but ectodermal surface cells are absent and lateral staining is lost. (D) Xwnt-8 expression in a UV-irradiated gastrula-stage embryo. (E-H) Early gastrulae (stage 10). Arrows represent the position of dorsal blastopore lips. Illustrated are Xwnt-8 expression in embryos injected ventrally with Wnt-1 (E) or goosecoid RNA (H) during cleavage stages and goosecoid expression in unperturbed (F) and Wnt-l-injected (G) embryos. Image published in: Christian JL and Moon RT (1993) Copyright © 1993. Image reproduced on with permission of the Publisher, Cold Spring Harbor Laboratory Press. This is an Open Access article. Gene Synonyms Species Stage(s) Tissue wnt8a.L wnt-8, wnt8, Xwnt-8, Xwnt8 X. laevis Sometime during NF stage 11 to NF stage 20 mesoderm Image source: Published Larger Image Printer Friendly View

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