Xenbase Image ID: 135308

Fig. 2. dHAND expression in staged zebrafish embryos. Whole-mount in situ hybridization (A–F) revealed expression of dHAND in the lateral plate mesoderm (lpm), pre-cardiac mesoderm (pcm), branchial arches (ba) and in the heart (ht). As early as 8 h post fertilization (hpf), expression was evident circumferentially in the anterior lateral plate mesoderm (alpm) and posterior lpm (plpm) in an oblique head-on view (A); positions of head (hd), eye (e) and tailbud (tb) are shown. At 12 hpf expression was again seen in the alpm and plpm (B); embryo is oriented with the head to the left and the tailbud to the right. Notochord (n) and yolk (y) are also noted. Expression in the pcm became evident at 18 hpf (C) and persisted into the heart tube at 24 hpf (D) seen ventrally, when branchial arch expression also became apparent. The embryo at 24 hpf demonstrated bilaterally symmetric expression of dHAND in the lateral plate mesoderm (E) in a dorsal view of the caudal region. Branchial arch expression persisted at 36 hpf (F) in the rostral region as seen dorsally. Transverse sections of 18 (G) and 24 (H) hpf embryos in the rostral and caudal regions, respectively, confirmed expression in the pcm and lpm. (I) demonstrates branchial arch expression of zdHAND at the histological level. (J) Shows expression of zdHAND throughout the heart tube. Image published in: Angelo S et al. (2000) Copyright © 2000. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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