XB-IMG-135356
Xenbase Image ID: 135356
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Figure 5. Quantification of trypsin mRNA at different developmental stages plotted with the known endogenous thyroid hormone
level (Leloup and Buscaglia 1977). Each point is the average of four different measurements that were reproducible within a factor of
two. The mRNA was quantified by slot-blot hybridization of total RNA from ovary, 6-month-old frog, or different stage tadpoles with
known amounts of PR22 cDNA as a standard and PR22 eDNA as a probe. The hybridization signal was measured by Cerenkov
counting. (Insert) The trypsin mRNA increase plotted at stages 62, 64, and 66 and frog {ng mRNA/mg total RNA) on an expanded
scale. The amount of RNA used was determined by UV absorption and verified after blotting by methylene blue staining (Herrin and
Schmidt 1988}. Standardization was also monitored by hybridization with the eDNA PR28 whose mRNA does not change (within
threefold) in abundance during development from ovary to frog (data not shown). Image published in: Shi YB and Brown DD (1990) Copyright © 1990. Image reproduced on with permission of the Publisher, Cold Spring Harbor Laboratory Press. This is an Open Access article. Larger Image Printer Friendly View |