XB-IMG-135359
Xenbase Image ID: 135359
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Figure 8. Developmental regulation of elastase and carboxypeptidase mRNA content. Ten micrograms of total RNA from ovary,
tadpoles at stages 41, 44/45, 54, 58, and 62, and 6-month-old small frog was used in lanes 1-7, respectively. The Northern filters were
hybridized with rat elastase I cDNA (A} and rat carboxypeptidase B cDNA (B) overnight in 5 x Denhardt's solution, 5 x SSPE, 1% SDS,
and 100 ~g/ml denatured salmon sperm DNA at 60~ The hybridized filters were washed twice with 0.5 x SSC, 0.1% SDS, at 50~
for 25 min. Image published in: Shi YB and Brown DD (1990) Copyright © 1990. Image reproduced on with permission of the Publisher, Cold Spring Harbor Laboratory Press. This is an Open Access article. Larger Image Printer Friendly View |