Xenbase Image ID: 135396

Fig. 1. Ectopic hh suppresses pronephric tubule and duct development but not glomeral development in Xenopus embryos. (A) Ectopic hh mRNA was delivered to the animal pole of either one (unilateral) or two (bilateral) cells at the two-cell stage. This results in the mRNA being present in the epidermis overlying the pronephric primordium, not the primordium itself. (B) Embryos injected unilaterally appear superficially normal at stage 42/43. Tadpoles that develop from bilaterally injected embryos lack all pronephric tubules and die from edema. (C, H, and E) Counterstained transverse section through 3G8 stained stage 38/39 embryo. 3G8 stains the apical surface of proximal pronephric tubules on the left. No tubules are present on the hh-injected side (right). A normal glomus is present on both sides of the injected embryo. (D) Transverse section through unilaterally injected embryo. As in C, the uninjected side has both tubules and glomus, but the injected side only has glomeral tissue. In place of tubules, a flat layer of mesoderm is observed (*). (E) Left side (uninjected) of hh unilaterally injected embryo probed with Na+K+ ATPase. Proximal tubules (white arrow), distal tubule, and duct (green arrow) are present. (F) As for panel E, except the probe is lim-1. (G) As for panel E, except staining is immunofluorescence with 3G8. (H) The opposite (right) side of the same embryo as shown in panel E. Note the complete absence of all pronephric tubules. (I) The opposite (right) side of the same embryo as shown in panel F. Note the complete absence of all lim-1-positive tissues. (J) The opposite (right) side of the same embryo as shown in panel G. No 3G8-positive tubules are present. Image published in: Urban AE et al. (2006) Copyright © 2006. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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