Xenbase Image ID: 135403

Fig. 8. The MEK inhibitor U0126 blocks pronephric tubule development. (A, B) Untreated control embryos at stage 32/33 stained for Na+K+ ATPase. Expression of Na+K+ ATPase is much stronger in the pronephros (white arrows) than it is in the otic vesicle (red arrows). (C, D) U0126-treated (from stage 14/15 to stage 32) embryos stained for Na+K+ ATPase. Strong expression is retained in the otic vesicle, but no pronephric staining is observed. (E, F) Stage 39 control embryos stained for Na+K+ ATPase (E) and NKCC2 (F) expression. (G, H) 50 μM U0126-treated embryos (from stage 12 to stage 39) stained for Na+K+ ATPase (G) and NKCC2 (H) expression. Very faint expression of both markers is visible (white arrows). This embryo was fixed, processed, and stained under identical conditions to the embryo shown in panels E and F. Image published in: Urban AE et al. (2006) Copyright © 2006. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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