XB-IMG-135404
Xenbase Image ID: 135404
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Fig. 9. FGF8-MO blocks the pronephric mesenchymal to epithelial transition. Normal epithelialization and acquisition of form in tailbud embryos visualized via Pax8 fluorescent in situ hybridization (A, B) or SEM (C) are illustrated in the top row. Stage 25 illustrates the mesenchymal stage of pronephric development, and stage 30 illustrates the early epithelialization stage. Test embryos were microinjected with FGF8-MO (14 ng) plus FLDx into blastomere V2.2 then raised to stage 30 or 33, fixed and stained for either Pax8 with a purple substrate (D–I) or 3G8 using a TRITC-coupled secondary antibody (J–L). The pronephric primordium forms (panels E and H) but fails to epithelialize. Note how the MO-injected pronephroi at stage 30 resemble the control pronephroi at the stage 25 mesenchymal period whereas the contralateral control side resembled the epithelialized controls shown in panels B and C. In both MO-injected examples, the lineage tracer (blue) is located in the pronephric mesenchyme (F, I, K, L) and is not present in the overlying somites. In panels H, I, and K, some epithelialization is occurring in the portion of the primordium that lack the tracer and the FGF8-MO. (M) Confocal optical transverse section through FGF8-MO unilateral stage 30/31 embryo stained for Pax8 via FISH. The left control side shows a Pax8-positive pronephros beginning to epithelialize (note forming lumen, white arrow), whereas the right side shows the FGF8-MO containing primordium as a Pax8-positive mesenchymal mass. Image published in: Urban AE et al. (2006) Copyright © 2006. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View |