XB-IMG-135434
Xenbase Image ID: 135434
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Fig. 2. Detection by RNAase
protection assay of TRa- and fi
transcripts in different regions
and tissues of Xenopus
tadpoles and froglets at
different developmental stages
before, during and after
metamorphosis. 10 (C) or 20
(A,B) ng of total RNA from
whole tadpoles (stages 28, 39,
44, 48), the head (h), middle
(m) and tail (t) portions of
tadpoles, hindlimbs (li) of
froglets and liver (1) of adult
Xenopus were hybridized to
lxlCPctsmin"1 of 32P-labelled
cRNA probes (Pr) for (A)
TRa- cRNA, (B) TR/3 or (C)
cardiac actin cRNA.
Unhybridized RNA and probe
were digested with RNAase A
and Tl and the protected
fragments resolved by
electrophoresis on sequencing
gels with appropriate size
markers (Zinn et at. 1985).
tRNA was used as a negative
control (not shown) and the
complete digestion of the
unprotected probe was verified
for each run. Autoradiograms
were exposed for 16 h for A
and B and for 30min for C.
Arrow indicates the cRNA
probe lengths and the expected
size of the protected probes
(260, 261 nt, respectively) for
TRa-, TR0 CRN As (see Fig. 1).
The multiple protected bands
of actin cRNA probe are
indicated by small arrows in C. Image published in: Kawahara A et al. (1991) Copyright © 1991. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View |