XB-IMG-135435
Xenbase Image ID: 135435
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Fig. 3. Dark-field imaging of localization by in situ
hybridization of TR mRNA in different tissues of stage 44
(premetamorphosis) and 55 (prometamorphosis) Xenopus
tadpoles. Sagittal sections were prepared from different
regions of tadpoles hybridized to a mixture of antisense
35S-labelled cRNA probe 3 (1x10^5 cts min-1 uL-1), as
shown in Fig. 1, unhybridized probe digested with RNAase
and the distribution of transcripts visualized by
autoradiography. Sense probes gave virtually no
hybridization and the autoradiographs are not shown here.
(A) Head region of stage 44 tadpole; (B) middle or trunk
region, stage 44; (C) tail, stage 44; (D) trunk and tail with
hindlimb bud in stage 55 (prometamorphosis). Left and
right sides of each image are the ventral and dorsal sides of
the tadpoles, respectively. Bright-field micrographs were
also prepared (not shown) and used for identifying the
different organs and tissues for which the abbreviations are
as follows: Br, brain; Ct, connective tissue; Gi, gill; He,
heart; Hlb, hindlimb bud; In, intestinal epithelium; Ki,
kidney; Li, liver; Mu, muscle; No, notochord; Pf,
pharyngeal floor epithelium; Ph, pharyngeal cavity; Sc,
spinal chord; Sk, skinj.Tf, tail fin; Tm, tail muscle. Note
that pigmented layers (Pi) and food particles (Fp) appear
as bright zones, also seen with sense probe controls.
Autoradiograms exposed for 9 days. Image published in: Kawahara A et al. (1991) Copyright © 1991. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.
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