Xenbase Image ID: 135473

Figure 1. Non-cell-autonomous induction of Nkx2.5 and Tbx5 by Gsk3β and dnTCF3. (A) Embryos were injected into one ventral blastomere at the 16-32-cell stage with mRNAs encoding Gsk3β or dnTCF3 along with Texas Red lysinated dextran (TRLDx, 10,000 MW) as a lineage label. The normally noncardiogenic VMZ mesendoderm was explanted at the onset of gastrulation (stage 10.25-10.5), cultured until cardiac tissue expressed Nkx2.5 and Tbx5 (stage 23-25), then stained for gene expression by in situ hybridization (blue). (B-E) Examples of non-cell-autonomous gene induction on histological section. Injection of GSK3β or dnTCF3 mRNAs (with TRLDx) induced Tbx5 (B,D) and Nkx2.5 (C,E). In situ hybridization is shown in the left panels, TRLDx fluorescence in the middle panels, and merged images on the right panels. Note that Tbx5- and Nkx2.5-positive cells were localized at a distance from lineage-labeled cells. Bar in B represents 20 μM. Image published in: Foley AC and Mercola M (2005) Copyright © 2005. Image reproduced on with permission of the Publisher, Cold Spring Harbor Laboratory Press. This is an Open Access article. Larger Image Printer Friendly View

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