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Fig. 5. Rarγ2 mRNA rescues posterior Hox and PSM expression in Rarγ2 MO embryos. (A-H) Embryos injected unilaterally at 2- or 4-cell stage. Injected side is indicated by magenta β-gal lineage tracer. (A,E) 5 ng Rarγ2.1 MO+5 ng Rarγ2.2 MO+control (mCherry) mRNA diminishes Hoxc10 and Msgn1 expression, curving the embryo axis in 100% of embryos (Hoxc10, 23/23; Msgn1, 13/13). (B,C,F,G) Co-injection of Rarγ2 MO and 1 ng Rarα2 mRNA or 1 ng Rarβ2 does not rescue the phenotype; however, (D,H) 1 ng Rarγ2 mRNA partially rescues axial curvature and Hoxc10 (18/23) and Msgn1 (23/35) expression. Tailbud embryos shown in dorsal view with anterior to left.
Image published in: Janesick A et al. (2014)
Copyright © 2014. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.
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