Xenbase Image ID: 135564

Fig. 6. c-SMRT overexpression knocks down posterior Hox, PSM and CNH markers. Embryos injected unilaterally at 2- or 4-cell stage with 4 ng c-smrt mRNA or control (mCherry) mRNA. Injected side indicated by magenta β-gal lineage tracer. (A,C,E,G,I,K) Control expression of Hoxc10, Hoxd10, Hoxc13, Msgn1, Tbx6 and xNot. (B,D,F,H,J,L) c-smrt overexpression shortens the axis on injected side in 70% of embryos. (B) c-smrt mRNA results in lateral knockdown (13/23 embryos), neural knockdown (7/23) or neural rostral shift (7/23) in Hoxc10 expression. (D) c-smrt mRNA produces neural and lateral knockdown (15/19) or lateral knockdown alone (4/19) of Hoxd10 expression. (F,H,J) c-smrt mRNA knocks down expression of Hoxc13 (14/18), Msgn1 (12/14) and Tbx6 (15/15). Tailbud embryos shown with anterior to left. (H′,J′) Caudal views of H and J. (L) c-smrt mRNA knocks down xNot (12/15) expression in neurula stage embryos (caudal view, dorsal to top). Image published in: Janesick A et al. (2014) Copyright © 2014. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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