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Fig. 8. Overexpression of DN-Rarγ2 mRNA expands expression of PSM and CNH markers, shifting or knocking down somitomere markers Thyl2 and Ripply2. (A-J) Embryos injected unilaterally at 2- or 4-cell stage. Injected side is indicated by magenta β-gal lineage tracer. (A,C,E,G,I) Control (mCherry) mRNA does not alter expression of Tbx6, Msgn1, Thyl2, Ripply2 or xNot. (B,D,F) 2 ng DN-Rarγ2 mRNA expands expression of Msgn1 (8/11) and Tbx6 (15/23) (green lines) and rostrally shifts xNot (8/10). (H,J) DN-Rarγ2 overexpression produces multiple phenotypes of Thyl2 and Ripply2 expression, as characterized and scored in K. Neurula embryos shown in dorsal view with anterior to left.
Image published in: Janesick A et al. (2014)
Copyright © 2014. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.
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