XB-IMG-135570
Xenbase Image ID: 135570
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Figure 2.
Overexpression of PKCδ lacking the catalytic domain inhibits gastrulation movements. (A) PKCδ1 lacking the catalytic domain (PKCδδC) inhibits gastrulation movements. RNA (100 pg) encoding PKCδδC was injected into the two dorsal blastomeres of four-cell embryos. PKCδδC severely inhibited gastrulation movements, and this effect was rescued by the coinjection of 1 ng of RNA encoding full-length PKCδ1. Embryos in the top panels are at the early neurula stage. (B) In situ hybridization of early gastrula embryos probed with a pan-mesodermal marker, Xbra, and dorsal mesodermal markers chordin (chd) and goosecoid (gsc). (Left) Uninjected. (Middle) PKCδδC RNA (200 pg) was injected into all four blastomeres of four-cell embryos. (Right) In order to trace the cell lineage, mRNA encoding β-galactosidase (β-gal) with a nuclear localization signal was coinjected with PKCδδC into two dorsal blastomeres of four-cell embryos. Cells expressing β-gal were stained in red. (C) Immunostaining of the notochord and somites in PKCδδC-injected embryos. (D) The PKCδδC mutant blocked the elongation of animal cap explants by activin. Activin RNA (0.5 pg) was injected with or without 100 pg of PKCδδC RNA. (E) The induction of mesodermal markers by activin in animal caps was not affected by PKCδδC. ui, uninjected; Em, whole embryo; gsc, goosecoid; chd, chordin. Image published in: Kinoshita N et al. (2003) Copyright © 2003. Image reproduced on with permission of the Publisher, Cold Spring Harbor Laboratory Press. This is an Open Access article.
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