Xenbase Image ID: 135599

Figure 4. Both TAD1 and the C-terminal activation domain (TAD2) of MAM are required for Notch signaling in injected Xenopusembryos. Xenopus embryos at the two-cell stage were injected with RNA encoding wild-type (Full-length) or mutant human MAM proteins (HMMδ74–301; HMM1–301) along with lacZ RNA as a tracer. The embryos were fixed and stained for β-galactosidase expression and double-labeled by whole-mount in situ hybridization for a neural-specific tubulin. Notch signaling was assessed by counting the number of primary neurons on the neural plate for the half of the embryo that was injected (inj) relative to that for the uninjected control (cont). (m) Midline; (i) interneuron; (l) lateral neuron. Image published in: Fryer CJ et al. (2002) Copyright © 2002. Image reproduced on with permission of the Publisher, Cold Spring Harbor Laboratory Press. This is an Open Access article. Gene Synonyms Species Stage(s) Tissue tubb2b.S N-Tub, n-tubulin, NBT, neural beta-tubulin, NST, ntubulin, Xn-tubulin X. laevis Throughout NF stage 15 interneuron neuron Rohon-Beard neuron motor neuron Image source: Published Larger Image Printer Friendly View

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