XB-IMG-135676
Xenbase Image ID: 135676
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FIG. 2. Injection of xWT1-KTS mRNA inhibits the development of the pronephric tubules. Antibody 3G8 was used to detect the
pronephric tubules (dark purple in all panels), and X-Gal was used to detect the b-galactosidase (light blue/green in B and D). (A and B)
Opposite sides of an experimental embryo injected with 1 ng of xWT1-KTS mRNA (10.25 ng of b-galactosidase mRNA). The uninjected
side (A) is normal, while all distal branches of the collecting tubule are absent on the injected side (B). The diameter and length of the
remaining common tubule and collecting tubule are also reduced on the injected side (B) compared to the uninjected side (A). The control,
uninjected pronephros (diagrammed in a9) is normal and is assigned 5 points; the pronephros on the injected side of the same embryo
(diagrammed in b9) is lacking all distal branches of the connecting tubule and is assigned only 2 points. The resulting PNI for this embryo
is 3, indicating a significant inhibition of pronephric development (see Table 1). (C and D) Opposite sides of a different embryo injected with
1 ng of xWT1-KTS mRNA (10.25 ng of b-galactosidase mRNA) displaying a more severe phenotype. The uninjected pronephros (C) is
normal and is assigned 5 points (c9). However, the pronephros on the injected side (D) consists only of a single unbranched tubule and is
assigned only 1 point (d9). The PNI for this embryo is 4, again indicating inhibition of pronephric development. While the morphology of
the uninjected pronephros is normal (c9), note that it is somewhat swollen compared to normal, likely the result of hypertrophy in order
to compensate for loss of pronephric function on the injected side (Fox, 1956; Howland, 1921). The reduction in the amount of
b-galactosidase staining in injected embryos (B and D) compared to the embryo shown in Fig. 1 is consistent with the amount of
b-galactosidase mRNA injected: 0.25 ng co-injected with xWT1 in this figure compared to 1 ng when b-galactosidase was injected alone in
Fig. 1. Image published in: Wallingford JB et al. (1998) Copyright © 1998. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View |