XB-IMG-135678
Xenbase Image ID: 135678
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FIG. 4. Injected xWT1 message persists throughout pronephric
development. RT–PCR using primers which specifically amplify
the injected message indicates that the injected xWT1 mRNA
remains stable until at least stage 30, by which time the pronephros
is well differentiated. EF1a was also amplified as a loading control.
Numbers at the top of the figure indicate developmental stages; 1,
injected embryos; 2, uninjected embryos; 2RT, no reverse transcriptase;
plasmid, PCR performed on 1 ng of xWT1 plasmid as a
positive control. The small amount of signal in the stage 10.5
uninjected sample is probably due to spillage during gel loading. Image published in: Wallingford JB et al. (1998) Copyright © 1998. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View |