XB-IMG-135735
Xenbase Image ID: 135735
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Figure 2. The secreted Wnt ligands Xwnt8
and mWnt8 induce neural tissue of posterior
character, whereas Wnt signal transduction
components induce neural tissue of
both anterior and posterior character. Onecell
Xenopus embryos were injected into the
animal hemisphere with 0.5 ng of mDbcatenin
(B,I), 1 ng of dnGSK3 (C,J), 1 ng of
dnXwnt8 (D,K), 0.5 ng of mWnt8 (E,L), and
0.5 ng of Xwnt8 (F,M). At blastula stage ectoderm
was explanted and allowed to develop
until late-neurula stage (20). Aged ectoderm
and whole embryos were then
double stained by in situ hybridization for
the cement gland marker, XAG (red), and
the general neural marker, Nrp1 (blue) (A–
G) or single stained by in situ hybridization
for the hindbrain marker, krox-20 (blue) (H–
N). A and H are whole-embryo controls; G
and N are uninjected ectodermal explants. Image published in: Baker JC et al. (1999) Copyright © 1999. Image reproduced on with permission of the Publisher, Cold Spring Harbor Laboratory Press. This is an Open Access article. Larger Image Printer Friendly View |