XB-IMG-135736
Xenbase Image ID: 135736
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Figure 3. Wnt signaling in ectoderm inhibits the expression of
Bmp4. One-cell Xenopus embryos were injected into the animal
hemisphere with 0.5 ng of mWnt8 (A,F,K), 0.5 ng of mDbcatenin
(B,G,L), and 1 ng of Frzb (D,I). At blastula stage ectoderm
was explanted and aged until gastrula stage (10.5) or neurula
stage (20). Ectoderm was subsequently probed with digoxigenin-
labeled antisense-Bmp4 (A–D), Nrp1 (F–I), or muscle
actin (K–M), by in situ hybridization. E, J, and N are whole
embryos probed with Bmp4, Nrp1, and muscle actin, respectively.
C, H, and M are uninjected control ectodermal explants. Image published in: Baker JC et al. (1999) Copyright © 1999. Image reproduced on with permission of the Publisher, Cold Spring Harbor Laboratory Press. This is an Open Access article. Larger Image Printer Friendly View |