Xenbase Image ID: 135896

Fig. 6. Microinjection of crescent mRNA does not inhibit development of anterior neural structures. (A,B) Overexpression of crescent mRNA enlarges the expression of a dorso-anterior marker in whole embryos. Neurulae (stage 16) in anterior view after whole mount in situ hybridization with Otx-2 and krox-20. (A) Uninjected control; Otx-2 expression is seen in the prospective forebrain (fb) and midbrain (mb). The krox-20 stripes mark rhombomeres 3 and 5. (B) Embryo injected with 4 ng crescent mRNA into the animal pole; the Otx-2 fore- and midbrain domain is enlarged; note that the posterior expression is continuous and not interrupted by a gap as in the uninjected sample. The anterior krox-20 stripe in rhombomere 3 is wider and stripe 5 is missing; Otx-2 staining in anterior endoderm is decreased (C) crescent or frzb-1 in combination with chordin promote dorsalization of the ectoderm (neural induction) in the absence of meso- derm. Animal caps were excised from injected embryos at stage 9, cultured until stage 27 and assayed by RT-PCR. N-CAM is a pan-neural marker, a- actin is a marker for mesoderm induction, and EF-1a a loading control. Image published in: Pera EM and De Robertis EM (2000) Copyright © 2000. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

Return to previous page