Xenbase Image ID: 135898

Fig. 5. Overexpression of crescent, but not of frzb-1, results in cyclopia. (A± D) Tadpole embryos (stage 40) in anterior view. (A) Uninjected control embryo. Note the two lateral eyes (e) and nasal placodes (white arrow- heads), the medial mouth (m) and cement gland (cg). (B) Embryo after four radial injections with crescent RNA at 50 pg/blastomere. Note that the eyes are fused along the midline, as are the nasal placodes (white arrow- head); the cement gland is smaller and the mouth missing. (C) Embryo injected with crescent RNA at higher doses (100 pg/blastomere) showing a single eye vesicle. The nasal placode, mouth and cement gland are not formed. (D) Embryo after injection with frzb-1 RNA (100 pg /blastomere). The distance between the two lateral eyes and between the nasal cavities (white arrowheads) is increased, the mouth is broadened and the cement gland signi®cantly enlarged. (E±G) Lateral view of swimming tadpole embryos at stage 43. (E) Uninjected control. (F) Embryo radially injected with crescent RNA (200 pg total) with cyclopia and reduced pigmentation. (G) Embryo radially injected with frzb-1 RNA (400 pg total). Note the enlargement of the head and of the ventral ®ssure of the eye (coloboma), which is thought to result from an increase of anterior ventral midline structures. Image published in: Pera EM and De Robertis EM (2000) Copyright © 2000. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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