Xenbase Image ID: 135916

Figure 2. dnXwnt-8 inhibits gsc induction and axis duplication by Wnts. Development of tadpoles (A, C,E, G, anterior to the left) and gsc in situ hybridization of gastrula-stage embryos (B,D,F,H). Embryos were injected at the four-cell stage with -300 pg of control RNA (A-D) or dnXwnt-8 RNA (E-H) into each ventral blastomere and again at the 8- to 16-cell stage into the ventral marginal zone with -25 pg of RNA encoding Xwnt-8 (C-F) or -60 pg of A[3-catenin RNA (G,H). The RNA amounts were based on first establishing the lowest amount of each required to elicit a duplication of the axis in most embryos. Embryos injected with control RNA develop with a single head {A) and a single domain of gsc expression (B, indicated by arrowhead). When injection of control RNA is followed by a second injection of RNA encoding wild-type Xwnt-8, embryos develop with a duplication of the embryonic axis (C, two heads indicated by arrowheads) and with an ectopic domain of gsc expression (D, two gsc expression domains indicated by arrowheads). When dnXwnt-8 RNA injection precedes that of RNA encoding wild-type Xwnt-8, axis duplication is repressed; embryos develop with one head (E) and with a single domain of gsc expression (F, indicated by arrowhead). Injection of dnXwnt-8 RNA does not affect the axis duplicating activity of subsequently injected A[3-catenin RNA, and tadpoles develop with two heads (G, indicated by arrowheads) and with an ectopic domain of gsc expression (H, two gsc expression domains indicated by arrowhead). Note that dnXwnt-8 RNA injections specifically inhibit Wnt-induced axis duplication (see also Table 2). Image published in: Hoppler S et al. (1996) Copyright © 1996. Image reproduced on with permission of the Publisher, Cold Spring Harbor Laboratory Press. This is an Open Access article. Larger Image Printer Friendly View

Return to previous page