XB-IMG-135918
Xenbase Image ID: 135918
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Figure 4. dnXwnt-8 inhibits Xwnt-8 expressed after the blastula
stage. (A) RT-PCR analysis of gene expression in control
stage 12 animal explants for a control marker (histone H4),
a dorsal marginal zone marker (gsc), and a ventral marginal
zone marker (Xpo). Embryos were injected at the two-cell stage
into the animal pole of both blastomeres with -800 pg of
control RNA or dnXwnt-8 RNA (as indicated at top) and -80 pg
of CSKA Xwnt-8 plasmid DNA (where indicated at top), and
animal caps were explanted and treated with activin (where
indicated by + ) and subjected to analysis with RT-PCR. (B-D)
Histological sections through animal pole explants. Embryos
were injected at the two-cell stage into the animal pole of
both blastomeres with -200 pg of control RNA (B), with -200
pg of control RNA, and -30 pg of CSKA Xwnt-8 plasmid (C), or
-200 pg of dnXwnt-8 RNA and -30 pg of CSKA Xwnt-8 plasmid
(D). Animal pole explants were prepared at stage 8, then
cultured until sibling embryos reached stages 38-40. Note that
dnXwnt-8 RNA represses the formation of vesicular structures
induced by CSKA Xwnt-8 (see Table 3A). (E-G) Stage 40 embryos
(anterior to the left and dorsal on the top) that developed
from embryos injected at the four-cell stage into the marginal
zone of both dorsal blastomeres with -200 pg of control RNA
(E), -200 pg of control RNA mixed with -30 pg of CSKA
Xwnt-8 plasmid (F), or -200 pg of dnXwnt-8 RNA mixed with
-30 pg of CSKA Xwnt-8 plasmid (G). Note that dnXwnt-8 interferes
with the Xwnt-8-mediated repression of dorsoanterior
structures such as cement gland (indicated by small arrowheads}
and eyes (see also Table 3B). Image published in: Hoppler S et al. (1996) Copyright © 1996. Image reproduced on with permission of the Publisher, Cold Spring Harbor Laboratory Press. This is an Open Access article. Larger Image Printer Friendly View |