Xenbase Image ID: 135930

Fig. 1. CASZ1 interacts with CHD5. (A,B) Full-length CASZ1 (bait) interacts with a partial CHD5 protein (prey) in a yeast two-hybrid assay. Cells placed under –His/–Ade selection. (C) Schematic of the structure of CHD5 depicting predicted domains. (D) RT-PCR on cDNA derived from Xenopus whole-embryo lysate. Ef1a was used as a loading control. (E) Expression of Chd5 RNA by in situ analysis in early tadpole Xenopus embryos (stage 37). h, heart; pr, pronephros; e, eye; pa, pharyngeal arches. (F-M) Colocalization of CHD5 and CASZ1 protein in the nuclei of the developing Xenopus (stage 40) myocardium (m). White boxes in F-I correspond to the magnified regions in J-M. Scale bars: 500 μm in E; 50 μm in F-I; 5 μm in J-M. Image published in: Sojka S et al. (2014) Copyright © 2014. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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