Xenbase Image ID: 135931

Fig. 2. CHD5 is required for cardiac morphogenesis. (A,B) CHD5-depleted embryos (stage 37) exhibit smaller, improperly looped hearts that fail to undergo chamber formation. Lateral views of whole-mount antibody staining with anti-tropomyosin (Tmy) antibody. (C,D) CHD5-depleted hearts fail to develop fully formed chambers and have a thicker myocardial layer. Transverse sections of cardiac actin-EGFP transgenic (CA-TG) Xenopus embryos (stage 37). (E-M) 3D reconstructions of early tadpole Xenopus embryos (stage 37) stained with anti-tropomyosin antibody viewed laterally and posteriorly. CHD5-depleted hearts (H-M) fail to complete migration and fusion (H-J, 54%), or fail to undergo proper looping and chamber formation (K-M, 31%). Only 15% display normal looping. n>25 per condition, two biological replicates. v, ventricle; o, outflow tract; i, inflow tract. D, dorsal; A, anterior; V, ventral; P, posterior. Scale bars: 100 μm in A-D; 50 μm in E-M. Image published in: Sojka S et al. (2014) Copyright © 2014. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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