Xenbase Image ID: 135935

Fig. 6. CASZ1 and CHD5 are required for myocardial tissue adhesion prior to cardiac looping and chamber formation. (A-X) Control, CHD5-depleted and CASZ1-depleted CA-GFP embryos were examined for expression of the tight junction markers ZO-1 (A-L) and claudin-5 (M-X). Expression of both ZO-1 and claudin-5 is reduced and appears diffuse and unorganized in transverse sections of CHD5- and CASZ1-depleted hearts (stage 33). Phenotypes represented across multiple sampled embryos (n≥4). White boxes in A,B,E,F,I,J,M,N,Q,R,U,V correspond to magnified images in C,D,G,H,K,L,O,P,S,T,W,X, respectively. Merge represents ZO-1 or claudin-5 (red), DAPI (blue) and CA-GFP (green). Scale bars: 100 µm in A,B,E,F,I,J,M,N,Q,R,U,V; 20 µm in C,D,G,H,K,L,O,P,S,T,W,X. Image published in: Sojka S et al. (2014) Copyright © 2014. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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