Xenbase Image ID: 135936

Fig. 7. CHD5 is crucial for CASZ1 function. (A,B) The region of CASZ1 containing the minimal CHD5-interacting domain amino acid residues 785-998 was identified using a yeast two-hybrid assay. (C-N) Control (C-E) and CASZ1-depleted (F-H) Xenopus embryos compared with CASZ1-depleted embryos co-injected with mRNA encoding either full-length CASZ1 (I-K) or CASZ1δCID (L-N) at early tadpole stage (stage 37). Full-length CASZ1 partially rescued cardiac looping and chamber formation (v), and outflow tract (o) and inflow tract (i) formation, whereas CASZ1δCID failed to rescue any aspect of cardiogenesis. (O-Q) Three independent experiments (n>20 per condition) of embryos were scored for cardia bifida (O), degree of proper cardiac looping (P) and chamber formation (Q) using whole-mount staining with anti-tropomyosin antibody. Fisher's exact test was performed for significance: NS, not significant; *P=0.003; ***P<0.0001. Error bars represent calculated s.e.m. Scale bars: 500 μm in C,D, F,G,I,J,L,M; 100 μm in E,H,K,N. Image published in: Sojka S et al. (2014) Copyright © 2014. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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