Xenbase Image ID: 135954

Figure 2. MCC differentiation is blocked in the skin of E2f4δCT-expressing embryos. (A–C) Shown are confocal images of embryonic skin (stage 28) of control embryos (A) or embryos injected with RNA encoding E2f4 (B) or E2f4δCT (C). Embryos were also injected with RNA encoding mRFP (red) and Hyls1-GFP (green) to mark membranes and basal bodies/centrioles, respectively, and stained for cilia (blue). Cells extending one or two cilia are marked with an arrow in C. Different cell types are identified based on morphology and cilia staining as outer cells (OCs), MCCs, small intercalated cells (INCs), and ciliated cells (CCs). Bar, 10 μm. (D) Representation of different skin cell types in control or E2f4- or E2f4δCT-expressing embryos based on 12 fields (98 μm2) from six embryos. (E) Effects on the cell cycle can be indirectly read out by the size of outer cells (Stubbs et al. 2012). E2f4δCT significantly (P < 0.05) decreases average cell size based on 24 cells from six embryos, indicating that it weakly promotes cell division. (F) Basal body number in MCCs in control or E2f4- or E2f4δCT-expressing embryos. Basal body counts based on 10 to 15 MCCs taken from six embryos. In all graphs, error bars indicate SD, and values significantly different (P < 0.05) from controls based on a two-tailed t-test are marked (asterisks). Image published in: Ma L et al. (2014) Copyright © 2014. Image reproduced on with permission of the Publisher, Cold Spring Harbor Laboratory Press. This is an Open Access article. Larger Image Printer Friendly View

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