Xenbase Image ID: 135955

Figure 3. E2f4δCT and Gmnn block Multicilin-induced basal body assembly during MCC differentiation. (A–D) Shown are confocal images of the embryonic skin expressing Multicilin-HGR alone (A) or with E2f4 (B), E2f4δCT (C), or Gmnn (D). Multicilin-HGR activity was induced with DEX at stage 12 and embryos fixed at stage 28. Membranes are marked with mRFP (red), basal bodies with Hyls1-GFP (green), and cilia in blue. Arrows denote monociliated cells. Bar, 10 μm. (E) Basal body number induced by ectopic expression of Multicilin-HGR activity in the presence of E2f4 and E2f4δCT. (F) Basal body number or the number of ciliated cells per field induced by Multicilin-HGR alone or in the presence of Gmnn. Basal body counts based on 10 to 15 cells taken from six embryos. Error bars indicate SD, and values significantly different (P < 0.05) from controls based on a two-tailed t-test are marked (asterisks) Image published in: Ma L et al. (2014) Copyright © 2014. Image reproduced on with permission of the Publisher, Cold Spring Harbor Laboratory Press. This is an Open Access article. Larger Image Printer Friendly View

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