Xenbase Image ID: 135956

Figure 4. E2f4δCT inhibits centriole gene expression during MCC differentiation. (A) RNA-seq analysis of skin progenitors isolated from Xenopus embryos expressing Multicilin-HGR alone or with E2f4δCT. Skin progenitors were isolated at stage 10, treated with DEX at stage 11 to induce MCC differentiation, and then extracted for RNA 9 h later. GO terms (P < 0.01) for genes down-regulated by E2f4δCT with the highest significance for microtubule (MT)-associated structures, including centrioles. GO term analysis for genes up-regulated by E2f4δCT failed to find a significant term. (B) Tukey box plot showing fold changes in response to E2f4δCT in the background of Multicilin-HGR-induced differentiation for both centriole components based on the supplemented list generated by Azimzadeh et al. (2012) or for cell cycle genes (Kegg Pathway) (Supplemental Tables 2, 3) (C) Heat map showing log2 fold change in response to E2f4δCT in Multicilin-HGR samples. (D) RNA samples were generated as in A but then analyzed in triplicate for the expression of the indicated gene using quantitative RT–PCR. RNA levels are shown after normalization to ubiquitously expressed ornithine decarboxylase (odc) RNA and are set relative to a value of 1 for uninjected controls. Fold change in the presence and absence of E2f4δCT is indicated. Image published in: Ma L et al. (2014) Copyright © 2014. Image reproduced on with permission of the Publisher, Cold Spring Harbor Laboratory Press. This is an Open Access article. Larger Image Printer Friendly View

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