Xenbase Image ID: 135959

Figure 2. Examples of changes in gene expression of putative Six1 targets after knock-down of endogenous Six1 levels by injection of antisense morpholino oligonucleotides (Six1 MOs), increased levels of wild-type Six1 by mRNA injection (Six1 WT), activation of Six1 targets by mRNA injection of an activating construct (Six1-VP16), or repression of Six1 targets by mRNA injection of a repressive construct (EnR-Six1) (see Brugmann et al., 2004 for validation of these constructs). Six1 MOs: Expression of Cdca7l in the segmenting somites (arrows on control [ctrl] side) is lost on the injected (inj) side (side views, anterior to left). Expression of HoxA3 in the neural crest (nc) is greatly reduced on the injected side, whereas hindbrain (hb) expression is maintained (side views, anterior to left). Expression of Ism1 is lost in the midbrain (arrow) (frontal view, dorsal to the top). Expression of IMAGE 3399268 is expanded in the neural plate (line depicts width from midline to lateral border) on the injected side (dorsal view, anterior to the bottom). Expression patterns of Cnfn1-a in the hatching gland (arrows) and of Snail1 in the migrating neural crest are reduced and disrupted on the injected sides (frontal views, dorsal to the top). Six1 WT: Expression of BG022063 is broader in the neural plate (line) and neural crest (bracket) on the injected side (frontal view, dorsal to the top). Expression of Nfya is broader in the neural tube (line) on the injected side (dorsal view, anterior to the top). Expression of IMAGE 3399268 in the segmented somites (arrows, ctrl side) is lost on the injected side (side views, anterior to left). Expression of IMAGE 4057931 in the neural crest (bracket), otocyst (oto) and trigeminal ganglion (arrow) is greatly diminished on the injected side (side views, anterior to left). Six1-VP16: Expression of Frizzled10b, Pbx1, and Zic2 in the neural plate is expanded (line) on the injected side (frontal views, dorsal to the top). EnR-Six1: Expression of Frizzled10b in the neural plate is reduced (line) on the injected side (frontal view, dorsal to the top). Expression of IMAGE 4057931 is reduced in the PPR (arrows on the control side; frontal view, dorsal to the top). Expression of Zic2 and Otx1 in the neural tube is reduced (line) on the injected sides (arrows; frontal views, dorsal to the top). In MO-injected embryos, injected sides were confirmed by the use of lysamine-labeled MOs (not shown). In mRNA-injected embryos, injected sides were confirmed by the presence of nuclear β-galactosidase lineage labeling (pink dots visible in most images). Image published in: Yan B et al. (2015) Copyright © 2015. Image reproduced with permission of the Publisher, John Wiley & Sons. Gene Synonyms Species Stage(s) Tissue septin5.S sep1, sept5, septin 1 X. laevis Throughout NF stage 26 otic vesicle cranial placode septin5.S sep1, sept5, septin 1 X. laevis Sometime during NF stage 11 to NF stage 14 preplacodal ectoderm cnfn.1.L cnfn.1-a, cnfn.1-b, cornifelin, plac8l2 X. laevis Throughout NF stage 20 hatching gland nfya.S cbf-a, cbf-b, hap2, nf-ya X. laevis Sometime during NF stage 18 to NF stage 20 neural plate neural tube cdca7l.S ram2 X. laevis Throughout NF stage 24 somite hoxa3.S hox1e, hoxa-3, hoxa3a X. laevis Throughout NF stage 24 hindbrain cranial neural crest lsm1.L X. laevis Throughout NF stage 20 midbrain fam89a.L X. laevis Throughout NF stage 20 paraxial mesoderm snai1.S LOC108702592, sna, snail, snail1, xSna, Xsnail X. laevis Throughout NF stage 26 cranial neural crest fam89a.L X. laevis Throughout NF stage 26 somite fzd10.S frizzled-10, frizzled10, Fz-10, fz10, fzd10-a, fzd10-b, Xfr9, Xfz10 X. laevis Throughout NF stage 20 neural plate neural tube zic2.L hpe5, xzic2, zic2-a, zic2-b, Zinc finger protein of the cerebellum 2 X. laevis Throughout NF stage 20 neural tube optic vesicle otx1.L xotx1 X. laevis Throughout NF stage 20 neural tube Image source: Published Larger Image Printer Friendly View

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